Renilla Luciferase Assay System E2820 from Promega

Product Specs
ItemRenilla Luciferase Assay System
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberE2820
ReactivityRenilla
Detection TargetLuciferase
SpeciesRenilla
Quantity1,000 assays
MethodLuciferase
Sample Typemammalian cells
Sensitivity10^–19 moles of Renilla luciferase detectable.

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Promega

2800 Woods Hollow Rd.

Madison, Wisconsin

United States

Phone: 800-356-9526
Fax: 800-356-1970

Company Profile

Website: http://www.promega.com

(1) A high-throughput Gaussia luciferase reporter assay for screening potential gasdermin E activators against pancreatic cancerActa Pharmaceutica Sinica BOctober 1, 2023Yang Liu, Xiaowei Zhang, Ping Zhang, Tingting He, Weitao Zhang, Dingyuan Ma, Ping Li, Jun ChenM-PER™ Mammalian Protein Extraction Reagent (78501) was purchased from Thermo Scientific (Boston, MA, USA). Renilla Luciferase Assay System (E2820) was purchased from Promega Corporation (Madison, WI, USA). Lipopolysaccharides (O55:B5) (L6529), etoposide

(2) Identification of an Arylnaphthalene Lignan Derivative as an Inhibitor against Dengue Virus Serotypes 1 to 4 (DENV-1 to -4) Using a Newly Developed DENV-3 Infectious Clone and RepliconMicrobiology spectrumAugust 17, 2023Hu M, Li WF, Wu T, Yang Y, Chen G, Chen T, Liu Y, Mei Y, Wu D, Wei Y, et al.The replication of the DENV-3 replicon was examined using a Renilla luciferase assay system (Promega; E2820) following the manufacturer’s instructions. Transformation and purification of plasmids. Plasmid transformation

(3) The AP2/ERF transcription factor TOE4b regulates photoperiodic flowering and grain yield per plant in soybeanPlant Biotechnology JournalAugust 1, 2023Haiyang Li, Haiping Du, Zerong Huang, Milan He, Lingping Kong, Chao Fang, Liyu Chen, Hui Yang, Yuhang Zhang, Baohui Liu, et al.using a Luciferase 1000 Assay System (Promega, E4550) and a Renilla Luciferase Assay System (Promega, E2820), respectively. The final transcriptional activity was expressed as the ratio between LUC and REN activities.

(4) The PRMT5/WDR77 complex restricts hepatitis E virus replicationPLOS PathogensJune 5, 2023Xiaohui Ju, Yanying Yu, Wenlin Ren, Lin Dong, Xianbin Meng, Haiteng Deng, Yuchen Nan, Qiang Dingluciferase assays Gaussia luciferase activity was measured using Renilla Luciferase Assay System (E2820, Promega). Collected supernatant was lysed with 5×lysis buffer for 15 minutes. Then, 10 μl sample was

(5) The E2F4/p130 Repressor Complex Cooperates with Oncogenic ΔNp73α To Inhibit Gene Expression in Human Papillomavirus 38 E6/E7-Transformed Keratinocytes and in Cancer CellsmSphereApril 20, 2023Valerio Taverniti, Hanna Krynska, Assunta Venuti, Marie-Laure Straub, Cécilia Sirand, Eugenie Lohmann, Maria Carmen Romero-Medina, Stefano Moro, Alexis Robitaille, Luc Negroni, et al.transfection, cells were washed with 50 μL of PBS and lysed with 40 μL of Renilla lysis buffer (Promega, E2820) for 30 min with agitation. Gaussia princeps luciferase enzymatic activity was measured using a Berthold

(6) A zinc finger transcription factor tunes social behaviors by controlling transposable elements and immune response in prefrontal cortexbioRxivApril 7, 2023Natalie L. Truby, R. Kijoon Kim, Gabriella M. Silva, Xufeng Qu, Joseph A. Picone, Rebecca Alemu, Rachael L. Neve, Xiaohong Cui, Jinze Liu, Peter J. HamiltonReport using the CellTiter-Glo 2.0 Assay (Promega #G9242) and Renilla luciferase assay system (Promega #E2820). Relative Luminesce Unit (RLU) was measured by the BMG-Omega plate reader using the Luminescence endpoint

(7) The MEF2A transcription factor interactome in cardiomyocytesCell Death & DiseaseApril 5, 2023Amira Moustafa, Sara Hashemi, Gurnoor Brar, Jörg Grigull, Siemon H. S. Ng, Declan Williams, Gerold Schmitt-Ulms, John C. McDermottaddition of Firefly Luciferase assay substrate (E1501, Promega) or Renilla Luciferase assay substrate (E2820, Promega). Firefly Luciferase values were normalized to the corresponding Renilla luciferase activity

(8) A bat MERS-like coronavirus circulates in pangolins and utilizes human DPP4 and host proteases for cell entryCellFebruary 16, 2023Chen J, Yang X, Si H, Gong Q, Que T, Li J, Li Y, Wu C, Zhang W, Chen Y, et al.Pro Universal SYBR qPCR Master Mix,Vazyme,Cat#: Q712 Renilla Luciferase Assay System,Promega,Cat#: E2820 MGIEasy RNA Library Prep Set,MGI,Cat#: 1000006384 MGISEQ-2000RS High-throughput Fast Sequencing Reagent

(9) SERTAD3 induces proteasomal degradation of ZIKV capsid protein and represents a therapeutic targetJournal of Medical VirologyFebruary 4, 2023Nina Sun, Rong‐Rong Zhang, Guang‐Yuan Song, Qiaomei Cai, Saba R. Aliyari, Karin Nielsen‐Saines, Jae U. Jung, Heng Yang, Genhong Cheng, Cheng‐Feng Qincells were lysed, and the levels of luminescence were measured using a Renilla luciferase assay kit (E2820; Promega) and a microplate reader (Bio-Tek) following the instructions of the manufacturers.30 2.9

(10) Human papillomavirus E6/E7 oncoproteins promote radiotherapy-mediated tumor suppression by globally hijacking host DNA damage repairTheranosticsJanuary 31, 2023Diane Bruyere, Patrick Roncarati, Alizee Lebeau, Thomas Lerho, Florian Poulain, Elodie Hendrick, Charlotte Pilard, Celia Reynders, Marie Ancion, Margaux Luyckx, et al.cells were washed and then incubated for 30 min at room temperature with the Renilla lysis buffer (E2820, Promega, Madison, WI, USA). After injection of 50 µl of luciferase substrate reagent in each well,

(11) Near-Infrared Imaging of Steroid Hormone Activities Using Bright BRET TemplatesInternational Journal of Molecular SciencesDecember 30, 2022Sung-Bae Kim, Ryo Nishihara, Ramasamy PaulmuruganThe reason for the reduction in the NLuc activity upon lysing the cells in this lysis buffer (cat. E2820, Promega) is due to the acidic pH level of the applied buffer. 2.3. Microslides Highlight Luciferase

(12) RNF185 regulates proteostasis in Ebolavirus infection by crosstalk between the calnexin cycle, ERAD, and reticulophagyNature communicationsOctober 12, 2022Zhang J, Wang B, Gao X, Peng C, Shan C, Johnson SF, Schwartz RC, Zheng YH.p1, and p2 were lysed to quantify virus production by Renilla-Glo® Luciferase Assay System (Promega, E2820). Authentic EBOV infection Huh7 cells grown in 24-well plates were transfected with vectors expressing

(13) Structure-function analysis of enterovirus protease 2A in complex with its essential host factor SETD3Nature communicationsSeptember 8, 2022Peters CE, Schulze-Gahmen U, Eckhardt M, Jang GM, Xu J, Pulido EH, Bardine C, Craik CS, Ott M, Gozani O, et al.Luciferase Assay lysis buffer. Luciferase expression was measured using a Renilla Luciferase Assay system (E2820; Promega) to determine the presence of infection. Luciferase reporter virus (CV-B3-RLuc)-synchronised

(14) Discovery of Novel 3-Hydroxyquinazoline-2,4(1H,3H)-Dione Derivatives: A Series of Metal Ion Chelators with Potent Anti-HCV ActivitiesInternational Journal of Molecular SciencesMay 25, 2022Yang Cao, Abudumijiti Aimaiti, Zeyun Zhu, Lu Zhou, Deyong YeBiomed Ltd., Shanghai, China). The cells in each well were then lysed with 50 μL of 1× lysis buffer (E2820, Promega, Madison, WI, USA). Subsequently, 50 μL of 1× luciferase assay substrate (E2820, Promega, Madison,

(15) Sites of vulnerability in HCV E1E2 identified by comprehensive functional screeningCell ReportsMay 24, 2022Jennifer M. Pfaff-Kilgore, Edgar Davidson, Kathryn Kadash-Edmondson, Mayda Hernandez, Erin Rosenberg, Ross Chambers, Matteo Castelli, Nicola Clementi, Nicasio Mancini, Justin R. Bailey, et al.Chemiluminescent Substrate,ThermoFisher Scientific,Cat# 34580 Renilla-Glo Luciferase assay,Promega,Cat# E2820 Pierce Femto kit,Pierce,Cat# 34095 Imaging System,Bio-Rad,ChemiDoc ,, Deposited data,Deposited data,Deposited

(16) Traditional Chinese Medicine Pien-Tze-Huang Inhibits Colorectal Cancer Growth and Immune Evasion by Reducing β-catenin Transcriptional Activity and PD-L1 ExpressionFrontiers in PharmacologyFebruary 3, 2022Qiang Chen, Yilin Hong, Shihe Weng, Peng Guo, Bei Li, Yong Zhang, Chundong Yu, Shicong Wang, Pingli Moin Harvest buffer and luciferase activity was determined using the luciferase reporter assay system (E2820, Promega). Transfection efficiency was normalized by Renilla luciferase activity. Western Blot Cell

(17) Human sorting nexin 2 protein interacts with Influenza A virus PA protein and has a negative regulatory effect on the virus replicationMolecular Biology ReportsJanuary 1, 2022Tuğba Koçmar, Elif Çağlayan, Erkan Rayaman, Kyosuke Nagata, Kadir TuranRenilla luciferase activities in the lysates were determined using a commercial kit (Promega # E1501 and E2820). The enzyme activity was measured as luminesces in a luminometer (GloMax20/20 Luminometer, Promega

(18) FoxO-KLF15 pathway switches the flow of macronutrients under the control of insuliniScienceDecember 17, 2021Yoshinori Takeuchi et al.chemicals,432-40201 KAPA SYBR Fast qPCR kit,NIPPON Genetics,KK4602 Renilla Luciferase Assay System,Promega,E2820 PrimeSTAR Mutagenesis Basal Kit,TAKARA Bio,R046A ReverTra Ace qPCR RT Master Mix,TOYOBO,FSQ-201 SuperPrep

(19) Bioorthogonal dissection of the replicase assembly of hepatitis C virusCell Chemical BiologySeptember 1, 2021Yang Zhang, Shuiye Chen, Zhenghong Yuan, Zhigang YiAnti-HA magnetic beads,ThermoFisher Scientific,Cat#88837; RRID:AB_2861399 Renilla luciferase assay system,Promega,Cat#E2820 MEGAscript T7 Transcription Kit,Invitrogen,Cat#AM1334 TransIT-LT1 Reagent,Mirus,Cat#MIR2306 TransIT-mRNA

(20) Genome-wide gene expression tuning reveals diverse vulnerabilities of M. tuberculosisCellAugust 19, 2021Barbara Bosch et al.Renilla luciferase assay as per manufacturer’s instructions (Promega Renilla Luciferase Assay system; #E2820). Luciferase activity was quantified in 96-well white plates (Costar; #3362) using a Spark multimode

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(1)

3/26/2018Renilla Luciferase Assay System Alex RayOur lab utilizes this system to detect HIV infection levels using virus that expresses luciferase. The assay system is very sensitive and easy to get up.
(1)

3/1/2017Renilla Luciferase Assay System Review Lana JagannathanOur lab uses HIV molecular clones that express Renilla luciferase reporter. We infect cells in vitro, or perform in vivo experiments where we infect our mice with these viruses. As a read out, we test... Read Review
(1)

2/27/2017Renilla Luciferase Assay Kit Nina FlerinOur laboratory investigates different ways to improve immune responses against HIV, including enhancement of the capacity of effector cells, such as NK cells, to kill HIV-infected cell. In our ... Read Review

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Renilla Luciferase Assay System from Promega

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Renilla Luciferase Assay System from Promega

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